英语翻译感激不尽,急用急用!Symptoms associated with the papaya ringspot virus (PRSV) were observed in papaya fields from eastern Cuba affected with the ‘Bunchy Top Symptom’ (BTS) phytoplasma.Total DNA and RNA extracted from symptomatic

英语翻译感激不尽,急用急用!Symptoms associated with the papaya ringspot virus (PRSV) were observed in papaya fields from eastern Cuba affected with the ‘Bunchy Top Symptom’ (BTS) phytoplasma.Total DNA and RNA extracted from symptomatic
英语翻译
感激不尽,急用急用!
Symptoms associated with the papaya ringspot virus (PRSV) were observed in papaya fields from eastern Cuba affected with the ‘Bunchy Top Symptom’ (BTS) phytoplasma.Total DNA and RNA extracted from symptomatic and asymptomatic papayas,were respectively subjected to nested PCR by using phytoplasma 16S rDNA primers,and to RT-PCR with generic coat-protein potyvirus primers.Nested PCR products were obtained for 45/47 of BTS-symptomatic papayas,as well as RT-PCR amplicons for 36/47.No PCR products were observed for asymptomatic plants.The BTS phytoplasma was identified as a member of group 16SrII,“Candidatus Phytoplasma aurantifolia”,while the potyvirus showed a 94% of CP identity with that of PRSV from Ivory Coast.Both typical phytoplasma pleomorphic bodies and potyvirus-like particles were observed in symptomatic papayas examined.Results suggest that the BTS phytoplasma and PRSV coexist in BTS-affected papayas,which must be considered for further management strategies,including investigations of further epidemiological implications.

英语翻译感激不尽,急用急用!Symptoms associated with the papaya ringspot virus (PRSV) were observed in papaya fields from eastern Cuba affected with the ‘Bunchy Top Symptom’ (BTS) phytoplasma.Total DNA and RNA extracted from symptomatic
与番木瓜环斑病毒（环斑病毒）有关的症状,观察到古巴东部与'束顶症状'（BTS）中病原木瓜领域的影响.分别提取有症状和无症状木瓜的DNA以及RNA,分别受到利用病原16S rDNA的巢式PCR引物,并以逆转录聚合酶链反应与普通外套蛋白病毒属引物.巢式PCR产物获得的基站45/47,症状木瓜,以及的RT - PCR扩增47分之36.没有PCR产物观察无症状植物.输血服务中心病原被确定为一组16SrII的成员,“Candidatus Phytoplasma aurantifolia”,而病毒属表明与环斑病毒,从象牙海岸与CP身份的94％.这两个典型的病原多形性机构和马铃薯Y病毒样颗粒在症状木瓜观察研究.结果表明,基站植原体和环斑病毒在基站共存影响木瓜,必须深入研究调查,以及进一步的流行病学调查的影响.