英语翻译Temporal regulation of shoot development in Arabidopsisthaliana by miR156 and its target SPL3Gang Wu and R.Scott Poethig*SPL3,SPL4 and SPL5 (SPL3/4/5) are closely related members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE family oftran

英语翻译Temporal regulation of shoot development in Arabidopsisthaliana by miR156 and its target SPL3Gang Wu and R.Scott Poethig*SPL3,SPL4 and SPL5 (SPL3/4/5) are closely related members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE family oftran
英语翻译
Temporal regulation of shoot development in Arabidopsis
thaliana by miR156 and its target SPL3
Gang Wu and R.Scott Poethig*
SPL3,SPL4 and SPL5 (SPL3/4/5) are closely related members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE family of
transcription factors in Arabidopsis,and have a target site for the microRNA miR156 in their 3\2 UTR.The phenotype of Arabidopsis
plants constitutively expressing miR156-sensitive and miR156-insensitive forms of SPL3/4/5 revealed that all three genes promote
vegetative phase change and flowering,and are strongly repressed by miR156.Constitutive expression of miR156a prolonged the
expression of juvenile vegetative traits and delayed flowering.This phenotype was largely corrected by constitutive expression of a
miR156-insensitive form of SPL3.The juvenile-to-adult transition is accompanied by a decrease in the level of miR156 and an
increase in the abundance of SPL3 mRNA.The complementary effect of hasty on the miR156 and SPL3 transcripts,as well as the
miR156-dependent temporal expression pattern of a 35S::GUS-SPL3 transgene,suggest that the decrease in miR156 is responsible
for the increase in SPL3 expression during this transition.SPL3 mRNA is elevated by mutations in ZIPPY/AGO7,RNA DEPENDENT
RNA POLYMERASE 6 (RDR6) and SUPPRESSOR OF GENE SILENCING 3 (SGS3),indicating that it is directly or indirectly regulated by
RNAi.However,our results indicate that RNAi does not contribute to the temporal expression pattern of this gene.We conclude
that vegetative phase change in Arabidopsis is regulated by an increase in the expression of SPL3 and probably also SPL4 and SPL5,
and that this increase is a consequence of a decrease in the level of miR156.
KEY WORDS:miRNA,Heterochrony,Phase change
翻译个大概意思就好

英语翻译Temporal regulation of shoot development in Arabidopsisthaliana by miR156 and its target SPL3Gang Wu and R.Scott Poethig*SPL3,SPL4 and SPL5 (SPL3/4/5) are closely related members of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE family oftran
时序调控的拟南芥拍mir156和发展目标spl3吴刚、钢筋poethig*斯科特 spl3、藻 14、spl5(spl3/4/5)息息相关的荔枝推动者成员结合蛋白像一家人拟南芥转录因子,四有一个目标,在现场为自己的微mir1563UTR区.拟南芥型 植物组成表达mir156敏感和mir156敏感spl3/4/5形式显示所有三个阶段的变化和植物基因促进开花,mir156和强烈的压抑.构表达mir156a长时间表达少年性状和延迟开花植物.这个构型,主要是表达了纠正mir156敏感spl3形式.少年到成年的过渡是伴随着跌幅水平和mir156 加丰spl3基因.匆忙的互补效应和mir156spl3勘验、 以及mir156依赖性表达模式有时空的35S::的GUS-spl3转,建议减少mir156负责spl3表达增加,在此过渡.spl3zippy/ago7突变基因是由高架、 依赖RNA的RNA聚合酶6(rdr6)3、抑癌基因沉默(不含) 说明它直接或间接调控RNA干扰.但是,我们的结果显示RNA干扰无助于这一基因时空表达模式.我们认为相变植物人拟南芥均受增加表达spl3大概 还spl4和spl5,这一增幅是下降的后果mir156水平.关键词:自然资源,heterochrony,相变