作业帮关于DNA的几种酶的疑问DNA的连接酶,解旋酶,聚合酶作用点分别是什么?（是碱基还是磷酸二酯键或者其他）效果是什么?1L还是没回答解旋酶和聚合酶的作用位置
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关于DNA的几种酶的疑问DNA的连接酶,解旋酶,聚合酶作用点分别是什么?（是碱基还是磷酸二酯键或者其他）效果是什么?1L还是没回答解旋酶和聚合酶的作用位置
关于DNA的几种酶的疑问
DNA的连接酶,解旋酶,聚合酶作用点分别是什么?（是碱基还是磷酸二酯键或者其他）效果是什么?
1L还是没回答解旋酶和聚合酶的作用位置
关于DNA的几种酶的疑问DNA的连接酶,解旋酶,聚合酶作用点分别是什么?（是碱基还是磷酸二酯键或者其他）效果是什么?1L还是没回答解旋酶和聚合酶的作用位置
连接酶的作用是将3’-OH 和5’端磷酸基团连接,所以是形成磷酸二酯键；
解旋酶的作用是使DNA的双螺旋结构断开为两条单链,所以它作用的是碱基间的氢键；
聚合酶是将游离的核糖核苷酸或者脱氧核糖核苷酸5’端磷酸与延伸的链中的3’-OH 相连接,形成磷酸二酯键.
解旋：复制时解开双螺旋链， 聚合：复制时连接游离的脱氧核苷酸，连接酶：连接目的基因链上的磷酸二酯键，
In molecular biology, DNA ligase is a special type of ligase that can link together two DNA strands that have double-strand break (a break in both complementary strands of DNA). That, a single-strand break, is fixed by a different type of DNA ligase using the complementary strand as a template but still requires DNA ligase to create the final phosphodiester bond to fully repair the DNA. Helicases are able to move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e. DNA, RNA, or RNA-DNA hybrid) using energy derived from ATP hydrolysis. Most helicases catalyze processive duplex unwinding. The primary function of a polymerase is the polymerization of new DNA or RNA against an existing DNA or RNA template in the processes of replication and transcription. In association with a cluster of other enzymes and proteins, they take nucleotides from solvent, and catalyse the synthesis of a polynucleotide sequence against a nucleotide template strand using base-pairing interactions. DNA polymerase can add free nucleotides to only the 3’ end of the newly-forming strand. This results in elongation of the new strand in a 5'-3' direction.Therefore, they build a new phosphodiester bond between the chain or primer and a nucleotide.