0.1M,pH8.0的硼酸缓冲液怎么配制?0.1 M borate buffer (pH 8.0)

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0.1M,pH8.0的硼酸缓冲液怎么配制?0.1 M borate buffer (pH 8.0)
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0.1M,pH8.0的硼酸缓冲液怎么配制?0.1 M borate buffer (pH 8.0)
0.1M,pH8.0的硼酸缓冲液怎么配制?
0.1 M borate buffer (pH 8.0)

0.1M,pH8.0的硼酸缓冲液怎么配制?0.1 M borate buffer (pH 8.0)
直接用0.1M硼酸溶液滴加氢氧化钠到pH8.0
这样：
SODIUM BORATE BUFFER
50x Stock:20 g NaOH
pH to 8.0 with approx 120 g H3BO3 (boric acid powder)
bring to 1 L with dH2O
NOTE:This stock will precipitate over time,requiring heat to re-dissolve crystallized salts before dilution.
1x Working Buffer:20 ml of 50x stock
980 ml dH2O
NOTE:The pH of the working solution will be higher than that of the 50x stock
NOTE:Do not re-use 1x solution for more than a day or it will go fuzzy.Really.
Translated to concentrations:
50x Stock:500 mM NaOH,pHed to 8.0 w/ H3BO3
1x Working buffer:10 mM NaOH,pHed to 8.5 w/ H3BO3

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