作业帮请问2×hyb solution是什么溶液?在做DNA分子杂交时用到的
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请问2×hyb solution是什么溶液?在做DNA分子杂交时用到的
请问2×hyb solution是什么溶液?在做DNA分子杂交时用到的
请问2×hyb solution是什么溶液?在做DNA分子杂交时用到的
2x Hybridisation Solution,不知道你用在什么地方,这是其中一种,用在Affymetrix Target Hybridisation
2X Hybridisation Buffer (Final 1X concentration is 100 mM MES, 1M [Na+], 20 mM EDTA, 0.01% Tween-20) 50 mL
8.3 mL of 12X MES Stock Buffer
17.7 mL of 5M NaCl
4.0 mL of 0.5M EDTA
0.1 mL of 10% Tween-20
19.9 mL of water
Do not autoclave. Store at 2°C to 8°C, and shield from light. Discard solution if yellow.
2X Hybridisation Buffer (Final 1X concentration is 100 mM MES, 1M [Na+], 20 mM EDTA, 0.01% Tween-20) 50 mL
8.3 mL of 12X MES Stock Buffer
17.7 mL of 5M NaCl
4.0 mL of 0.5M EDTA
0.1 mL ...
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2X Hybridisation Buffer (Final 1X concentration is 100 mM MES, 1M [Na+], 20 mM EDTA, 0.01% Tween-20) 50 mL
8.3 mL of 12X MES Stock Buffer
17.7 mL of 5M NaCl
4.0 mL of 0.5M EDTA
0.1 mL of 10% Tween-20
19.9 mL of water
Do not autoclave. Store at 2°C to 8°C, and shield from light. Discard solution if yellow.
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