英语翻译A bacterium Pseudomonas sp.KBEL 101 isolated from soil was immobilized within polyacrylamide gel and used for the synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin.The half-life of immobilized whole cell D-hydantoinase

英语翻译A bacterium Pseudomonas sp.KBEL 101 isolated from soil was immobilized within polyacrylamide gel and used for the synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin.The half-life of immobilized whole cell D-hydantoinase
英语翻译
A bacterium Pseudomonas sp.KBEL 101 isolated from soil was immobilized within polyacrylamide gel and used for the synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin.The half-life of immobilized whole cell D-hydantoinase was found to be about 50 hrs.In order to increase the operational stability of immobilized whole cell D-hydantoinase,a carbon or nitrogen source was supplied with the reaction mixture in the continuous stirred tank reactor.As a sole source of carbon,glycerol was found to be most effective,and the activity of immobilized whole cell enzyme was maintained stably during 7 days when 0.1%(W/V) glycerol solution was provided.In the case of nitrogen source,supplying of 0.1%(W/V) yeast extract prolonged the half-life of immobilized whole cell D-hydantoinase to about 25 days.
摘要翻译

英语翻译A bacterium Pseudomonas sp.KBEL 101 isolated from soil was immobilized within polyacrylamide gel and used for the synthesis of D-p-hydroxyphenylglycine from DL-5-substituted hydantoin.The half-life of immobilized whole cell D-hydantoinase
一种细菌假单胞菌.KBEL101从土壤中分离聚丙烯酰胺凝胶内固定和用于DP-对羟基苯甘氨酸,DL -5-取代乙内酰脲的合成.被发现的全细胞固定化D -海因酶的半衰期约50小时.为了提高全细胞固定化D -海因酶,反应混合物在连续搅拌反应釜提供一个碳或氮源运行的稳定性.作为碳的唯一来源,甘油是最有效的,并在7天提供了0.1％（W / V）甘油溶液时,固定化全细胞酶的活性,维持稳定.在氮源的情况下,提供0.1％（W / V）酵母提取全细胞固定化D -海因酶的半衰期延长25天左右.

一个细菌假单胞菌。kbel101是从土壤中分离固定在聚丙烯酰胺凝胶，用于合成对羟基苯甘氨酸从dl-5-substituted海因。半固定化细胞海因酶被发现约50小时。为了提高操作稳定性的固定化细胞海因酶，碳或氮源提供了反应混合物的连续搅拌反应釜。作为唯一碳源，甘油被发现是最有效的，和活动的固定化细胞酶稳定地保持在7天时0.1%（瓦特/五）甘油的解决方案是提供。在案件的氮源，提供0.1%（瓦特/五）...

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一个细菌假单胞菌。kbel101是从土壤中分离固定在聚丙烯酰胺凝胶，用于合成对羟基苯甘氨酸从dl-5-substituted海因。半固定化细胞海因酶被发现约50小时。为了提高操作稳定性的固定化细胞海因酶，碳或氮源提供了反应混合物的连续搅拌反应釜。作为唯一碳源，甘油被发现是最有效的，和活动的固定化细胞酶稳定地保持在7天时0.1%（瓦特/五）甘油的解决方案是提供。在案件的氮源，提供0.1%（瓦特/五）酵母提取物长期半衰期海因酶固定化细胞约25天。

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