英语翻译Pieces of lemon were placed on soil at different places and after a few days fungal growth appeared in each piece of lemon.The spores from each piece were transferred separately into PDA plates and incubated at 30℃.After 2 days growth a

英语翻译Pieces of lemon were placed on soil at different places and after a few days fungal growth appeared in each piece of lemon.The spores from each piece were transferred separately into PDA plates and incubated at 30℃.After 2 days growth a
英语翻译
Pieces of lemon were placed on soil at different places and after a few days fungal growth appeared in each piece of lemon.The spores from each piece were transferred separately into PDA plates and incubated at 30℃.After 2 days growth appeared in each plate.Spore suspensions were prepared from each plate
separately,diluted suitably,streaked on other PDA plates and incubated at 30℃ for 2 days.Spores from single isolated colonies were taken from each plate,transferred aseptically to PDA slants and incubated at 30℃ for 2 days.In all 400 isolates were obtained and stored at 4℃.
Filter paper disks in Petri dishes were sterilised and soaked with sterilised sucrose based medium containing universal indicator solution of the pH range 1-10.5.
Petri dishes were inoculated with spores of different isolates and incubated at 30℃for 3 days.Acid producing strains were detected by the formation of coloured (pink) zone around the colony.Zone diameter was taken as a criterion of good acid producing ability of isolates.Of these acid producers,citric acid producing
strains were identified by cultivating on bagasse moistened with sucrose based medium in 250 ml flasks for 8 days.Fermented material was extracted using distilled water and citric acid was estimated in extracts.Of these isolates,A.niger DS 1 was found to be the best citric acid producer and therefore selected for further work.

英语翻译Pieces of lemon were placed on soil at different places and after a few days fungal growth appeared in each piece of lemon.The spores from each piece were transferred separately into PDA plates and incubated at 30℃.After 2 days growth a
在来自不同地方的土壤上放置柠檬切片,几天后每片柠檬上都会出现霉菌.将每片柠檬上面的霉菌孢子分别转移到PDA（机率分布分析器）皿上并在30℃下培养.2天之后每个培养皿里的孢子都出现了增长.分别停止每个培养皿里孢子的增长,加以适当的稀释,然后在新的培养皿上铺成带状,在30℃下培养2天.从每个培养皿中取出单独隔离菌落的孢子,无菌地转移到PDA斜面皿并在30℃下培养2天.总共获得400个隔离菌落并在4℃下储藏.培养皿里面的滤纸片是无菌的,且在灭菌的含蔗糖的pH值范围1-10.5通用指示剂溶液中浸泡.不同菌落的孢子放到培养皿里面嫁接,并在30℃下储藏3天.产酸的菌株通过菌落周围颜色（粉色）的形成被检测出来.区域直径被用于衡量菌落产酸能力的标准.在这些产酸菌株当中,产柠檬酸的菌株通过在250毫升的长颈瓶中以蔗糖为主要介质的蔗渣里培养8天后鉴定出来.发酵材料用蒸馏水提取,柠檬酸估计在提取液里面.这些菌落里面,提取液中一种曲霉DS1被发现是最好的产柠檬酸菌株,因此被选为今后的研究课题.