PCR不能扩增出目的条带,用的是文献中提供的引物总是扩增不出来,目的片段279bp,我用的条件是94℃,5min;94℃,30s,50℃,30s,72℃,45s,30个循环;72℃,5min.其中退火温度有试过56℃,58℃,都不行,只有引
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![PCR不能扩增出目的条带,用的是文献中提供的引物总是扩增不出来,目的片段279bp,我用的条件是94℃,5min;94℃,30s,50℃,30s,72℃,45s,30个循环;72℃,5min.其中退火温度有试过56℃,58℃,都不行,只有引](/uploads/image/z/10261347-51-7.jpg?t=PCR%E4%B8%8D%E8%83%BD%E6%89%A9%E5%A2%9E%E5%87%BA%E7%9B%AE%E7%9A%84%E6%9D%A1%E5%B8%A6%2C%E7%94%A8%E7%9A%84%E6%98%AF%E6%96%87%E7%8C%AE%E4%B8%AD%E6%8F%90%E4%BE%9B%E7%9A%84%E5%BC%95%E7%89%A9%E6%80%BB%E6%98%AF%E6%89%A9%E5%A2%9E%E4%B8%8D%E5%87%BA%E6%9D%A5%2C%E7%9B%AE%E7%9A%84%E7%89%87%E6%AE%B5279bp%2C%E6%88%91%E7%94%A8%E7%9A%84%E6%9D%A1%E4%BB%B6%E6%98%AF94%E2%84%83%2C5min%EF%BC%9B94%E2%84%83%2C30s%2C50%E2%84%83%2C30s%2C72%E2%84%83%2C45s%2C30%E4%B8%AA%E5%BE%AA%E7%8E%AF%EF%BC%9B72%E2%84%83%2C5min.%E5%85%B6%E4%B8%AD%E9%80%80%E7%81%AB%E6%B8%A9%E5%BA%A6%E6%9C%89%E8%AF%95%E8%BF%8756%E2%84%83%2C58%E2%84%83%2C%E9%83%BD%E4%B8%8D%E8%A1%8C%2C%E5%8F%AA%E6%9C%89%E5%BC%95)
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