蛋白质氨基酸为什么具有紫外性吸收功能
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蛋白质氨基酸为什么具有紫外性吸收功能
蛋白质氨基酸为什么具有紫外性吸收功能
蛋白质氨基酸为什么具有紫外性吸收功能
蛋白质氨基酸中苯环和大pai键吸收紫外线
Proteins in solution absorb ultraviolet light with absorbance maxima at 280 and 200 nm. Amino acids with aromatic rings are the primary reason for the absorbance peak at 280 nm. Peptide bonds are prim...
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Proteins in solution absorb ultraviolet light with absorbance maxima at 280 and 200 nm. Amino acids with aromatic rings are the primary reason for the absorbance peak at 280 nm. Peptide bonds are primarily responsible for the peak at 200 nm. Secondary, tertiary, and quaternary structure all affect absorbance, therefore factors such as pH, ionic strength, etc. can alter the absorbance spectrum.
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蛋白质的紫外吸收主要是因为其中的
酪氨酸(含苯酚基团)
色氨酸(含吲哚基团)
苯丙氨酸(含苯环)
这些基团具有部分双键的性质